Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Generation of Human Alloantigen-specific T Cells…
JoVE Journal
Immunology and Infection

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Immunology and Infection
Generation of Human Alloantigen-specific T Cells from Peripheral Blood
 

Generation of Human Alloantigen-specific T Cells from Peripheral Blood

Article DOI: 10.3791/52257-v • 09:47 min • November 21st, 2014
November 21st, 2014 •
1, 1
1Department of Pathology, University of California, San Diego

Capítulos

Resumo
Automatic Translation

This article describes a method for the generation and propagation of human T cell clones that specifically respond to a defined alloantigen. This protocol can be adapted for cloning human T cells specific for a variety of peptide-MHC ligands.

Tags

Keywords: T Cells Alloantigen MHC HLA TCR Alloreactivity Graft Versus Host Disease Allograft Rejection Peripheral Blood Leukocytes PMHC Multimer In Vitro Culture T Cell Clones
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter