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JoVE Journal Neuroscience
Electrophysiological and Morphological Characterization of Neuronal Microcircuits in Acute Brain Slices Using Paired Patch-Clamp Recordings
 

Electrophysiological and Morphological Characterization of Neuronal Microcircuits in Acute Brain Slices Using Paired Patch-Clamp Recordings

Article DOI: 10.3791/52358-v • 10:24 min • January 10th, 2015
January 10th, 2015 •
1, 1, 1,2
1Institute of Neuroscience and Medicine (INM-2), Research Centre Jülich, 2Department of Psychiatry, Psychotherapy and Psychosomatics, Medical Faculty, JARA, RWTH Aachen University

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Resumo
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Patch-clamp recordings and simultaneous intracellular biocytin filling of synaptically coupled neurons in acute brain slices allow a correlated analysis of their structural and functional properties. The aim of this protocol is to describe the essential technical steps of electrophysiological recording from neuronal microcircuits and their subsequent morphological analysis.

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Keywords: Paired Patch-clamp Recording Acute Brain Slice Neuronal Microcircuits Synaptic Transmission Electrophysiology Morphology Connectivity Chemical Synapses Gap Junctions Neuron Reconstruction Dendritic Truncation Axonal Truncation
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