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对于外小泡的使用流式细胞仪分析技术
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JoVE Journal Biologia
Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry
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对于外小泡的使用流式细胞仪分析技术

DOI:
10.3791/52484-v

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09:39 min

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March 17, 2015

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, ,
1Blood Systems Research Institute, 2Department of Medicine,University of California, San Francisco, 3Department of Laboratory Medicine,University of California, San Francisco

Capítulos

  • 00:05Título
  • 01:23Individual Extracellular Vesicle Detection
  • 05:33Bead-based Detection of EVs and Exosomes
  • 07:19Results: Representative Individual and Bead-based Detection Data
  • 08:49Conclusion

Summary

Tadução automática

Tadução automática

使用流式细胞仪（FCM）外囊泡（电动车）的测量许多不同的方法存在。确定最合适的方法时使用的几个方面应予以考虑。两个协议用于测量电动车使用的是单独的检测或珠为基础的方法介绍。

Tags

Keywords: Extracellular VesiclesFlow CytometryCell-cell CommunicationBiological ProcessesAnalysis TechniquesEV ProcessingBead-based ApproachIndividual Detection MethodSignal-to-noise RatioBackground FluorescenceClinical SamplesExosomes

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