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在啮齿动物疟原虫原虫的体内评估和裂殖子入侵流式细胞仪

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

In Vivo Assessment of Rodent Plasmodium Parasitemia and Merozoite Invasion by Flow Cytometry

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在啮齿动物疟原虫原虫的体内评估和裂殖子入侵流式细胞仪

DOI:

10.3791/52736-v

•

07:16 min

•

April 05, 2015

•

Patrick M. Lelliott, Brendan J. McMorran, Simon J. Foote², Gaetan Burgio

¹Australian School of Advanced Medicine,Macquarie University, ²John Curtin School of Medical Research,Australian National University

Capítulos

00:05Título
01:11Red Blood Cell Labeling and Transfusion
03:07Blood Sample Collection and Preparation for Flow Cytometry
04:10Flow Cytometry
05:19Results: Parasitemia Measurement and Merozoite Invasion Assessment
06:32Conclusion

Summary

Tadução automática

疟原虫侵入和重复内红细胞。裂殖子入侵和原虫的准确评估，因此在评估疟疾感染的过程中是至关重要的。在这里，我们描述了一种流式细胞仪基础的协议用于疟疾的小鼠模型中，这些参数的测量。

Tags

Keywords: Plasmodium Parasitemia Malaria Flow Cytometry Hoechst JC-1 Red Blood Cells Howell-Jolly Bodies Merozoite Invasion In Vivo Assessment

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