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追踪药物诱导Colocalizational变化分析受体后内部贩运

JoVE Journal
Biologia

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JoVE Journal Biologia

Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis

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追踪药物诱导Colocalizational变化分析受体后内部贩运

DOI:

10.3791/52824-v

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07:48 min

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July 03, 2015

•

Edmund Ong², Catherine Cahill^2,3

¹Department of Anesthesiology and Perioperative Care,University of California Irvine, ²Department of Biomedical and Molecular Sciences,Queen’s University, ³Department of Pharmacology,University of California Irvine

Capítulos

00:05Título
01:25Drug Treatment of Cultured Cells and Immunocytochemistry
03:26Microscope Settings and Imaging
04:57Colocalization Analysis and Data Normalization
06:17Results: Representative Photomicrographs of Receptor and Compartment-marker Labeling with False-color Colocalization Maps
07:18Conclusion

Summary

Tadução automática

受体运输调节信号传导和细胞反应的配体和是，本身，响应于细胞的条件，包括配体诱导的信号传导。在这里，我们描述了一个强大而灵活的技术评估定量使用免疫标记和colocalizational分析药物诱导的受体贩卖。

Tags

Keywords: Receptor Trafficking Receptor Internalization Colocalization Analysis Immunolabeling Confocal Microscopy Drug-induced Changes Cell Signaling Intracellular Trafficking Quantitative Analysis

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