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Sporing lægemiddelinduceret Ændringer i Receptor Post-internalisering Trafficking af Colocalizational Analysis
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Biologia
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JoVE Journal Biologia
Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis

Sporing lægemiddelinduceret Ændringer i Receptor Post-internalisering Trafficking af Colocalizational Analysis

DOI:
10.3791/52824-v

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07:48 min

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July 03, 2015

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,
1Department of Anesthesiology and Perioperative Care,University of California Irvine, 2Department of Biomedical and Molecular Sciences,Queen’s University, 3Department of Pharmacology,University of California Irvine

Capítulos

  • 00:05Título
  • 01:25Drug Treatment of Cultured Cells and Immunocytochemistry
  • 03:26Microscope Settings and Imaging
  • 04:57Colocalization Analysis and Data Normalization
  • 06:17Results: Representative Photomicrographs of Receptor and Compartment-marker Labeling with False-color Colocalization Maps
  • 07:18Conclusion

Summary

Tadução automática

Tadução automática

Receptor menneskehandel modulerer signalering og celle lydhørhed over for ligander og er i sig selv reagerer på celle, herunder ligand-induceret signalering. Her beskriver vi en stærk og fleksibel teknik til kvantitativ vurdering af lægemiddelinduceret receptor menneskehandel hjælp immunolabeling og colocalizational analyse.

Tags

Keywords: Receptor TraffickingReceptor InternalizationColocalization AnalysisImmunolabelingConfocal MicroscopyDrug-induced ChangesCell SignalingIntracellular TraffickingQuantitative Analysis

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