Journal
/
/
Visualisatie van Chondrocyte Intercalatie en Directional Proliferatie via Zebrabow Klonale Cell Analysis in het Embryonale Meckel's Kraakbeen
JoVE Journal
Biologia do Desenvolvimento
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia do Desenvolvimento
Visualization of Chondrocyte Intercalation and Directional Proliferation via Zebrabow Clonal Cell Analysis in the Embryonic Meckel’s Cartilage
DOI:

06:40 min

October 21, 2015

, , ,

Capítulos

  • 00:05Título
  • 01:21Embryo Collection, Tamoxifen Treatment, and Embryo Selection
  • 02:46Mounting Embryos in Methylcellulose and Fluorescence Microscopy
  • 04:33Results: Chondrocyte Intercalation and Directional Proliferation in ubi:Zebrabow-M;sox10:ERT2-Cre Transgenic Zebrafish
  • 06:15Conclusion

Summary

Tadução automática

Cel organisatie van craniofaciale botten is al lang de hypothese, maar niet direct zichtbaar. Multispectrale cell labeling en in vivo levende beeldvorming maakt visualisatie van de dynamische cel gedrag in de zebravis onderkaak. Hier hebben we detail het protocol te manipuleren Zebrabow transgene vis en direct waar te nemen cel intercalatie en morfologische veranderingen van chondrocyten in de Meckel's kraakbeen.

Vídeos Relacionados

Read Article