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qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping
 

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

Article DOI: 10.3791/52941-v • 07:00 min • May 25th, 2015
May 25th, 2015 •
1, 1, 2, 1, 2, 1
1Department of Biochemistry and Molecular Pharmacology, Institute for Systems Genetics, 2Roche Life Science, USA

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Designer chromosomes of the Synthetic Yeast Genome project, Sc2.0, can be distinguished from their native counterparts using a PCR-based genotyping assay called PCRTagging, which has a presence/absence endpoint. Here we describe a high-throughput real time PCR detection method for PCRTag genotyping.

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QPCRTag Analysis High Throughput Real Time PCR Assay Sc2.0 Genotyping Synthetic Yeast Genome Project Designer Yeast Chromosomes Synthetic Chromosome In Vivo Function Validation PCRTags Open Reading Frames (ORFs) PCR Assay PCRTag Primers Amplicons Agarose Gel Electrophoresis Genome Size Real Time PCR-based Detection Assay QPCRTag Analysis Multiwell Plate
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