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Cardiac Pressure-Volume Loop Analysis Using Conductance Catheters in Mice
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Biologia
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JoVE Journal Biologia
Cardiac Pressure-Volume Loop Analysis Using Conductance Catheters in Mice

Cardiac Pressure-Volume Loop Analysis Using Conductance Catheters in Mice

DOI:
10.3791/52942-v

•

08:15 min

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September 17, 2015

•

,
1Department of Medicine,Duke University Medical Center

Capítulos

  • 00:05Título
  • 01:18Conductance Catheter Placement
  • 04:03Varying Afterload Using Transient Aortic Occlusion
  • 05:00Varying Preload Using Transient Inferior Vena Cava Occlusion
  • 05:57Saline and Cuvette Calibration
  • 07:05Results: Change in Contractility With Drug Administration
  • 07:46Conclusion

Summary

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Cardiac pressure-volume loop analysis is the most comprehensive way to measure cardiac function in the intact heart. We describe a technique to perform and analyze cardiac pressure volume loops, using conductance catheters.

Tags

Cardiac Pressure-volume Loop AnalysisConductance CathetersVentricular FunctionContractilityComplianceAfterloadPreloadMiceMicrosurgical TechniquesElectrical ConductanceVolume EstimationGenetic Mutant Mouse ModelsDrug Treatment StudiesHemodynamic MeasurementsData Analysis

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