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生物膜制作的溶剂辅助脂质双分子层（SALB）方法
JoVE Journal
Bioengenharia
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JoVE Journal Bioengenharia
Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer (SALB) Method
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生物膜制作的溶剂辅助脂质双分子层（SALB）方法

DOI:
10.3791/53073-v

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09:38 min

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December 01, 2015

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, , , , ,
1School of Materials Science and Engineering,Nanyang Technological University, 2Centre for Biomimetic Sensor Science,Nanyang Technological University, 3School of Chemical and Biomedical Engineering,Nanyang Technological University

Capítulos

  • 00:05Título
  • 01:22Formation of Supported Lipid Bilayer on a Hydrophilic Solid Support and Cholesterol-enriched Supported Membranes
  • 03:05Membrane Fluidity Assay
  • 05:01Cholesterol Quantification Assay
  • 07:51Results: Fabrication of Planar Bilayer on Solid Support Using SALB Method
  • 08:44Conclusion

Summary

Tadução automática

Tadução automática

我们提出了一个试验性协议，形成对固体基质支持的脂质双层不使用脂质囊。我们展示了一个一步法，以形成具有各种生物应用胆固醇富集域上的二氧化硅和金脂质双层以及支持膜。

Tags

Keywords: Biomembrane FabricationSolvent-assisted Lipid BilayerSALB MethodSupported Lipid BilayerSLBVesicle FusionLipid DepositionOrganic SolventHydrophilic SurfaceCholesterol-rich Membranes

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