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Capítulos
- 00:05Título
- 01:07Maintenance of Caco-2 Cells
- 02:24Stimulating Lipoprotein Secretion and Lipoprotein Isolation
- 04:35Transmission Electron Microscopy Analysis
- 05:42Results: Analysis of Lipoproteins Secreted by Caco-2 Cells
- 06:35Conclusion
Caco-2 cells can serve as an in vitro model to study the enterocyte transport of lipids, and lipid-soluble drugs/vitamins. The permeable membrane system separates the apical from the basolateral compartment, while the lentivirus expression system offers an effective gene overexpression method. The isolation of lipoproteins is confirmed by TEM.
