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成像APP的细胞内运输与光活化GFP

JoVE Journal
Biologia

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JoVE Journal Biologia

Imaging the Intracellular Trafficking of APP with Photoactivatable GFP

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成像APP的细胞内运输与光活化GFP

DOI:

10.3791/53153-v

•

07:55 min

•

October 17, 2015

•

Joshua H. K. Tam, Stephen H. Pasternak²

¹Department of Physiology and Pharmacology, Robarts Research Institute,Western University, ²Department of Clinical Neurological Sciences,Western University

Capítulos

00:05Título
00:54Cell Transfection and Microscope Stage Preparation
01:52Photoactivation of ROIs over the Golgi
03:20Determining the Downstream Compartment
04:00Ensuring Accuracy of Photoactivation within the Golgi
05:42Results: Photoactivatable Intracellular Trafficking Analysis Using βAPP-paGFP Photoactivation
07:21Conclusion

Summary

Tadução automática

而细胞表面蛋白的传输相对容易地研究，可视化细胞内蛋白质的贩卖是困难得多。在这里，我们使用结合光活化绿色荧光蛋白的结构和表现出的方法来准确地遵循从高尔基体向下游车厢淀粉样前体蛋白，并按照其清除。

Tags

Amyloid Precursor Protein (APP)Beta-amyloid (AÎ²)Alzheimer’s Disease Photoactivatable GFP (paGFP)Intracellular Trafficking Golgi Endosomes Lysosomes Secretase Inhibitors Pulse-chase Experiments

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