Journal
/
Biologia
/
Optical Mapping of Intra-Sarcoplasmic Reticulum Ca2+ and Transmembrane Potential in the Langendorff-perfused Rabbit Heart
JoVE Journal
Biologia
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia
Optical Mapping of Intra-Sarcoplasmic Reticulum Ca2+ and Transmembrane Potential in the Langendorff-perfused Rabbit Heart

Optical Mapping of Intra-Sarcoplasmic Reticulum Ca2+ and Transmembrane Potential in the Langendorff-perfused Rabbit Heart

DOI:
10.3791/53166-v

•

09:26 min

•

September 10, 2015

•

, ,
1Department of Pharmacology,University of California Davis, 2Departments of Pharmacology and Biomedical Engineering,University of California Davis

Capítulos

  • 00:05Título
  • 01:49Preparations
  • 03:31Harvesting, Perfusing and Dye-loading a Rabbit Heart
  • 07:09Optical Mapping
  • 07:56Resulting Vm and SR Ca2+ Signals from a Langendorff-perfused Rabbit Heart
  • 08:59Conclusion

Summary

Tadução automática

Tadução automática

This article describes the detailed protocol and equipment necessary for dual optical mapping of transmembrane potential (Vm) and free intra-sarcoplasmic reticulum (SR) Ca2+ in the Langendorff-perfused rabbit heart. This method allows for direct observation and quantification of Vm and SR Ca2+ dynamics in the intact heart.

Tags

Optical MappingSarcoplasmic ReticulumCa2+ HandlingTransmembrane PotentialLangendorff-perfused HeartFluo-5NExcitation-contraction CouplingArrhythmiaSpatial MappingDual Optical Mapping

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/53166?article_type=v

Proper Care and Cleaning of the Microscope
check_url/pt/53166?article_type=v

Optical Mapping of Langendorff-perfused Rat Hearts
check_url/pt/53166?article_type=v

Visualizing the Beating Heart in Drosophila
check_url/pt/53166?article_type=v

Semi-automated Optical Heartbeat Analysis of Small Hearts
check_url/pt/53166?article_type=v

Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube
check_url/pt/53166?article_type=v

Quantification of γH2AX Foci in Response to Ionising Radiation
check_url/pt/53166?article_type=v

Evaluation of the Spatial Distribution of γH2AX following Ionizing Radiation
check_url/pt/53166?article_type=v

Clonogenic Assay: Adherent Cells
check_url/pt/53166?article_type=v

Voltage and Calcium Dual Channel Optical Mapping of Cultured HL-1 Atrial Myocyte Monolayer
check_url/pt/53166?article_type=v

High-resolution Optical Mapping of the Mouse Sino-atrial Node

Read Article