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Optisk Kortlægning af Intra-reticulum Ca<sup> 2+</sup> Og transmembrane potentiale i Langendorff-perfunderet kanin Heart
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Biologia
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JoVE Journal Biologia
Optical Mapping of Intra-Sarcoplasmic Reticulum Ca2+ and Transmembrane Potential in the Langendorff-perfused Rabbit Heart

Optisk Kortlægning af Intra-reticulum Ca<sup> 2+</sup> Og transmembrane potentiale i Langendorff-perfunderet kanin Heart

DOI:
10.3791/53166-v

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09:26 min

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September 10, 2015

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, ,
1Department of Pharmacology,University of California Davis, 2Departments of Pharmacology and Biomedical Engineering,University of California Davis

Capítulos

  • 00:05Título
  • 01:49Preparations
  • 03:31Harvesting, Perfusing and Dye-loading a Rabbit Heart
  • 07:09Optical Mapping
  • 07:56Resulting Vm and SR Ca2+ Signals from a Langendorff-perfused Rabbit Heart
  • 08:59Conclusion

Summary

Tadução automática

Tadução automática

Denne artikel beskriver den detaljerede protokol og nødvendigt udstyr til dobbelt optisk kortlægning af transmembranpotentiale (V m) og gratis intra-reticulum (SR) Ca2 + i Langendorff-perfunderet kanin hjerte. Denne metode giver mulighed for direkte observation og kvantificering af V m og SR Ca 2+ dynamik i den intakte hjerte.

Tags

Optical MappingSarcoplasmic ReticulumCa2+ HandlingTransmembrane PotentialLangendorff-perfused HeartFluo-5NExcitation-contraction CouplingArrhythmiaSpatial MappingDual Optical Mapping

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