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Water in Oil Emulsions: A New System for Assembling Water-soluble Chlorophyll-binding Proteins with Hydrophobic Pigments
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JoVE Journal Química
Water in Oil Emulsions: A New System for Assembling Water-soluble Chlorophyll-binding Proteins with Hydrophobic Pigments

Water in Oil Emulsions: A New System for Assembling Water-soluble Chlorophyll-binding Proteins with Hydrophobic Pigments

DOI:
10.3791/53410-v

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11:26 min

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March 21, 2016

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,
1Department of Biological Chemistry,Weizmann Institute of Science, 2Migal-Galilee Research Institute

Capítulos

  • 00:05Título
  • 00:49Prepare Chlorophyll a Stock Solutions
  • 04:11Prepare Organic and Aqueous Phases of Emulsion
  • 06:43Assembly of WSCP with Chlorophyll a in Emulsion
  • 09:05Results: Assembly of WSCP Apoproteins Using Water in Oil Emersions
  • 10:15Conclusion

Summary

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This manuscript describes a simple and high-throughput method for assembling water-soluble proteins with hydrophobic pigments that is based on water-in-oil emulsions. We demonstrate the effectiveness of the method in the assembly of native chlorophylls with four variants of recombinant water-soluble-chlorophyll binding proteins (WSCPs) of Brassica plants expressed in E. coli.

Tags

Water In Oil EmulsionsChlorophyll-binding ProteinsHydrophobic PigmentsChlorophyll ExtractionSpirulina PlatensisCyanobacteriaDEAE-SepharoseAcetoneMethanolDiethyl EtherProtein-pigment Complexes

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