É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
1Laboratory for Neurobiology and Gene Therapy, Department of Neurosciences,KU Leuven, 2Division of Nuclear Medicine,Leuven University Hospital – KU Leuven, 3Laboratory for Molecular Virology and Gene Therapy, Department of Pharmaceutical and Pharmacological Sciences,KU Leuven, 4Leuven Viral Vector Core,KU Leuven
Capítulos
- 00:05Título
- 01:11Stereotactic Injection of rAAV-α-SYN into the Rat Substantia Nigra
- 05:18Results: Neurodegeneration Induced by rAAV2/7 α-SYN
- 07:30Conclusion
This manuscript describes how viral vector-mediated local gene delivery provides an attractive way to express transgenes in the central nervous system. The protocol outlines all crucial steps to perform a viral vector injection in the substantia nigra of the rat to develop a viral vector-based animal model for Parkinson's disease.
