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An Improved Method for Rapid Intubation of the Trachea in Mice
 

An Improved Method for Rapid Intubation of the Trachea in Mice

Article DOI: 10.3791/53771-v • 08:46 min • February 22nd, 2016
February 22nd, 2016 •
1,2, 3, 2
1Department of Environmental and Occupational Health Sciences, University of Washington, 2Department of Medicine, Cedars-Sinai Medical Center, 3Division of Pulmonary and Critical Care Medicine, University of Washington

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This article presents a rapid and simple method for administering bleomycin directly into the mouse trachea via intubation. Key advantages of this method are that it is highly reproducible, easy to master, and does not require specialized equipment or lengthy recovery times.

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Keywords: Intubation Trachea Mouse Lung Administration Reagents Acute Lung Injury Fibrogenesis Sterilized Procedure Board Spirometer Isoflurane Anesthesia
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