Journal
/
/
Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
JoVE Journal
Biologia
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia
Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
DOI:

11:02 min

May 27, 2016

, , , , , ,

Capítulos

  • 00:05Título
  • 01:04In Silico Prediction of Aternative First Exons of Bcrp1 Using BLAST Analysis of Mouse EST Database
  • 04:30Design of Reporter Constructs for Bcrp1 E1U, E1A, E1B, and E1C Promoters
  • 07:55Reporter Assay Methods
  • 09:03Results: Evaluation of Bcrp1 Alternative Promoter Function
  • 10:07Conclusion

Summary

Tadução automática

With the murine ABC transporter Bcrp1 (Abcg2) as an example, in-silico protocols are presented to detect alternative promoter usage in genes expressed in mouse tissues, and to evaluate the functionality of the alternative promoters identified using reporter assays.

Vídeos Relacionados

Read Article