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Immunology and Infection

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JoVE Journal Immunology and Infection
Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents
 

Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents

Article DOI: 10.3791/53846-v • 14:04 min • March 25th, 2016
March 25th, 2016 •
1, 1
1Immunity, Microenvironment, Virus, Cancer Research Center in Lyon

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Neutrophils are the most abundant type of white blood cells. The isolation of neutrophils from human blood by density gradient separation method and the differentiation of human promyelocytic (HL60) cells along the granulocytic pathway are described here; to test their role on sensitivity of lymphoma cells to anti-lymphoma agents.

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Keywords: Neutrophil Isolation Lymphoma Cell Sensitivity Density Gradient Centrifugation EDTA-treated Blood Primary Leukemic Cells Granulocytes Erythrocytes Red Blood Cell Lysis Innate Immune System Cancer Therapy Three-dimensional Cell Culture
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