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研究Wnt信号传导过程中的航空公司图案

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Studying Wnt Signaling During Patterning of Conducting Airways

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研究Wnt信号传导过程中的航空公司图案

DOI:

10.3791/53910-v

•

13:00 min

•

October 16, 2016

•

John Snowball, Manoj Ambalavanan, Debora Sinner²

¹Neonatology and Pulmonary Biology-Perinatal Institute,Cincinnati Children’s Hospital Medical Center, ²University of Cincinnati, College of Medicine

Capítulos

00:05Título
01:20Whole Mount X-galactosidase Staining
05:23Lectin Staining
06:18Whole Mount Immunofluorescence Staining and Confocal Microscopy
07:43Cell Proliferation
09:37Results: Wnt Signaling and Developing Tracheal and Lung Tissue
11:28Conclusion

Summary

Tadução automática

利用耦合到整装报告小鼠和切片染色，显微镜和体内试验方便的呼吸道的正常图案形成底层机制的分析。在这里，我们将介绍这些技术如何促进Wnt信号的气管发育过程中的分析。

Tags

Keywords: Wnt Signaling Conducting Airways Patterning Pulmonary Biology Cell Proliferation Mesenchymal Condensations Tracheal Cartilage Genetically Engineered Mouse Models Lung Explants Tracheas E11 Embryos Laparotomy Uterine Horns Embryonic Membranes Esophagus 4% Paraformaldehyde X-gal Staining

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