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LC-MS Analysis of Human Platelets as a Platform for Studying Mitochondrial Metabolism
 

LC-MS Analysis of Human Platelets as a Platform for Studying Mitochondrial Metabolism

Article DOI: 10.3791/53941-v • 06:04 min • April 4th, 2016
April 4th, 2016 •
*1,2, *2,3, 1,2, 2,3, 2,3, 4, 4, 1,2, 5, 1,2
1Center for Cancer Pharmacology, University of Pennsylvania, 2Center for Excellence in Environmental Toxicology, University of Pennsylvania, 3Penn SRP and Department of Systems Pharmacology and Translational Therapeutics, University of Pennsylvania, 4Division of Traumatology, Department of Surgery, Critical Care and Acute Care Surgery, University of Pennsylvania, 5A.J. Drexel Autism Institute, Drexel University
* These authors contributed equally

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Here we show isolated human platelets can be used as an accessible ex vivo model to study metabolic adaptations in response to the complex I inhibitor rotenone. This approach employs isotopic tracing and relative quantification by liquid chromatography-mass spectrometry and can be applied to a variety of study designs.

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LC-MS Analysis Human Platelets Mitochondrial Metabolism Xenobiotic Treatment Disease State Cellular Metabolism Intervention Transformed Cell Lines Human Health Rotenone Dimethyl Sulfoxide Serial Dilution Tyrode's Buffer Solution DMSO Vehicle Control Isolation Of Platelets Whole Blood Samples Centrifuge
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