Journal
/
Imunologia e Infecção
/
A RAPID Method for Blood Processing to Increase the Yield of Plasma Peptide Levels in Human Blood
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
A RAPID Method for Blood Processing to Increase the Yield of Plasma Peptide Levels in Human Blood

A RAPID Method for Blood Processing to Increase the Yield of Plasma Peptide Levels in Human Blood

DOI:
10.3791/53959-v

•

11:36 min

•

April 28, 2016

•

, , , , , , , , ,
1Charité Center for Internal Medicine and Dermatology, Division General Internal and Psychosomatic Medicine,Charité Universitätsmedizin Berlin, Campus Benjamin Franklin, 2Department of Internal Medicine, Institute of Neurogastroenterology and Motility,Martin-Luther Hospital, Academic Teaching Institution of Charité Universitätsmedizin Berlin, 3Department of Hepatology and Gastroenterology, Molecular Cancer Research Center (MKFZ), Campus Virchow-Klinikum,Charité-Universitätsmedizin Berlin

Capítulos

  • 00:05Título
  • 00:59Blood Processing
  • 03:14Recovery of Radiolabled Peptides
  • 05:15High Performance Liquid Chromatography (HPLC) and Radiolabled Ghrelin
  • 06:50Radioimmunoassay
  • 08:42Results: Comparison of RAPID Method Versus Standard Processing for Assessing Plasma Peptide Levels in Human Blood
  • 10:43Conclusion

Summary

Tadução automática

Tadução automática

The RAPID blood processing method can be used in humans and yields higher peptide levels as well as allows for assessment of the correct molecular form. Therefore, this method will be a valuable tool in peptide research.

Tags

Blood ProcessingRAPID MethodPlasma Peptide LevelsEndogenous Peptide HormonesBlood SamplingBlood CentrifugationRAPID BufferChromatography CartridgesRadioimmunoassayIodine 125 Radiolabeled Peptides

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/53959?article_type=v

Depletion of Specific Cell Populations by Complement Depletion
check_url/pt/53959?article_type=v

Isolation of Mouse Peritoneal Cavity Cells
check_url/pt/53959?article_type=v

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)
check_url/pt/53959?article_type=v

Introducing Shear Stress in the Study of Bacterial Adhesion
check_url/pt/53959?article_type=v

High-throughput Detection Method for Influenza Virus
check_url/pt/53959?article_type=v

Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols
check_url/pt/53959?article_type=v

Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry
check_url/pt/53959?article_type=v

A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood
check_url/pt/53959?article_type=v

Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages
check_url/pt/53959?article_type=v

A Simple Flow Cytometric Method to Measure Glucose Uptake and Glucose Transporter Expression for Monocyte Subpopulations in Whole Blood

Read Article