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التحقيق في وظيفة من Coronin (أ) في المجاعة الاستجابة المبكرة لل<em> Dictyostelium discoideum</em> من جانب تجميع فحوصات
JoVE Journal
Biologia
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JoVE Journal Biologia
Investigating the Function of Coronin A in the Early Starvation Response of Dictyostelium discoideum by Aggregation Assays
DOI:

07:48 min

June 18, 2016

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Capítulos

  • 00:05Título
  • 00:58Observe the Early Starvation Response of Dictyostelium discoideum by Time-lapse Microscopy
  • 02:14cAMP Pulsing of Dictyostelium discoideum Cells During Starvation
  • 03:54Induction of Dictyostelium discoideum Development Through Exposure to Conditioned Medium
  • 05:32Results: cAMP Pulses Rescue the Early Developmental Defect of corA- Dictyostelium discoideum
  • 06:45Conclusion

Summary

Tadução automática

The social amoeba Dictyostelium discoideum undergoes a developmental transition into a multicellular organism when starved. The evolutionary conserved protein coronin A plays a crucial role in the initiation of development. Using aggregation assays as our main method, we aim to elucidate the role of coronin A in early development.

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