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JoVE Journal Developmental Biology
Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation
 

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation

Article DOI: 10.3791/53978-v • 09:07 min • June 21st, 2016
June 21st, 2016 •
*1, *2, 1, 1,2,3
1Sanford-Burnham-Prebys Medical Discovery Institute at Lake Nona, 2Department of Biochemistry and Molecular Biology, Research Center for Molecular Medicine, Medical and Health Science Center, University of Debrecen, 3MTA-DE “Lendulet” Immunogenomics Research Group, University of Debrecen
* These authors contributed equally

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In this work we provide an experimental workflow of how active enhancers can be identified and experimentally validated.

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Keywords: Gene Regulatory Elements Enhancers Retinoic Acid Embryonic Stem Cell Differentiation Reporter Plasmid Construct Transfection Transcription Regulation
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