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预测与基因调控元件的验证激活过程中维甲酸诱导胚胎干细胞分化
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation
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预测与基因调控元件的验证激活过程中维甲酸诱导胚胎干细胞分化

DOI:
10.3791/53978-v

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09:07 min

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June 21, 2016

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, , ,
1Sanford-Burnham-Prebys Medical Discovery Institute at Lake Nona, 2Department of Biochemistry and Molecular Biology,Research Center for Molecular Medicine, Medical and Health Science Center, University of Debrecen, 3MTA-DE “Lendulet” Immunogenomics Research Group,University of Debrecen

Capítulos

  • 00:05Título
  • 00:45Enhancer Selection Based on ChIP-seq Analysis
  • 01:27Transfection of Embryonic Stem Cells
  • 03:21β-Galactosidase and Luciferase Assays
  • 05:19Characterization of Enhancer RNA and Measurement of eRNA Transcription
  • 06:34Results: Retinoic Acid Induced Enhancer Activation in Embryonic Stem Cells
  • 08:13Conclusion

Summary

Tadução automática

Tadução automática

In this work we provide an experimental workflow of how active enhancers can be identified and experimentally validated.

Tags

Gene Regulatory ElementsEnhancersRetinoic AcidEmbryonic Stem Cell DifferentiationReporter Plasmid ConstructTransfectionTranscription Regulation

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