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マウスの冠動脈内皮細胞の単離
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Biologia
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JoVE Journal Biologia
Isolation of Mouse Coronary Endothelial Cells
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マウスの冠動脈内皮細胞の単離

DOI:
10.3791/53985-v

•

07:36 min

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July 03, 2016

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, ,
1Department of Physiology,University of Arizona, Tucson

Capítulos

  • 00:05Título
  • 00:37Magnetic Bead Preparation
  • 02:02Dissection
  • 03:09Bead Conjugation, Cell Culture, and Imaging
  • 05:45Results: Representative Mouse Coronary Endothelial Cell (MCEC) Imaging
  • 06:13Conclusion

Summary

Tadução automática

Tadução automática

This protocol is prepared to share our method of isolating mouse coronary endothelial cells for the purpose of imaging or to conduct molecular biological experiments.

Tags

Keywords: Mouse Coronary Endothelial CellsCardiovascular ResearchCD31 BufferSheep Anti-rat IgG BeadsAnti-mouse CD31 AntibodyHeparinPentobarbitalKrebs BufferHBSSEndothelial Cell IsolationCell PurificationCell Yield

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