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Expression and Purification of Virus-like Particles for Vaccination
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Biologia
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JoVE Journal Biologia
Expression and Purification of Virus-like Particles for Vaccination

Expression and Purification of Virus-like Particles for Vaccination

DOI:
10.3791/54041-v

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06:17 min

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June 02, 2016

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, ,
1Center for Vaccines and Immunology, Department of Infectious Diseases, College of Veterinary Medicine,University of Georgia

Capítulos

  • 00:05Título
  • 00:58Influenza H3N2 Virus-like Particle (VLP) Generation
  • 02:10CHIK VLP Expression Using Baculovirus/Insect Cell System
  • 03:18Sedimentation and Purification of VLPs and SVPs
  • 04:42Results: Successful Expression and Purification of VLPs
  • 05:23Conclusion

Summary

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Here, we present a protocol for synthesizing virus-like particles using either baculovirus or mammalian expression systems, and ultracentrifugation purification. This highly customizable approach is used to identify viral antigens as vaccine targets in a safe and flexible manner.

Tags

Virus-like ParticlesVLPsVaccinationProtein ExpressionPurificationMammalian CellsInsect CellsTransfectionBaculovirusCHIKVAntigenVaccineSerologyDiagnosis

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