É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:05Título
- 00:43Collecting Skin Biopsies
- 02:49Surgical Denervation
- 05:36Behavioral Test of Denervation
- 06:22Results: Touch Dome Cells and Merkel Cells Require Innervation
- 07:20Conclusion
This article includes detailed protocols for genetic labeling of mouse skin, surgical denervation, skin biopsy and visualizing labeled epithelia by whole-mount β-galactosidase staining. These methods can be used to test the requirement for nerves in mouse models of normal and pathological skin.
