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Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses
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Biologia
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JoVE Journal Biologia
Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses

Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses

DOI:
10.3791/54161-v

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07:42 min

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July 13, 2016

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, , ,
1Department of Medicine,Stony Brook University School of Medicine, 2Department of Physiology & Biophysics,Stony Brook University School of Medicine

Capítulos

  • 00:05Título
  • 00:48Colon and Small Intestine Preparation
  • 04:38Swiss Rolling
  • 05:55Results: Stained Swiss Roll Intestinal Preparations
  • 07:09Conclusion

Summary

Tadução automática

Tadução automática

Accurate identification and location of epithelial cells along the intestinal mucosal lining are essential to define different cell lineages. Proper imaging of intestinal tissues is crucial for identification of protein expression patterns with maximum resolution. This study aims to delineate the optimal methods and conditions for processing mouse intestinal tissues.

Tags

ImmunohistochemicalImmunofluorescentSwiss Rolling TechniqueFixation MethodMouse IntestineAbdominal MusclesColonSmall IntestineCecumModified Bouin’s FixativeGavage Needle

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