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Biologia do Desenvolvimento
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体细胞重编程的动力学测量和实时可视化
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Kinetic Measurement and Real Time Visualization of Somatic Reprogramming
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体细胞重编程的动力学测量和实时可视化

DOI:
10.3791/54190-v

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08:56 min

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July 30, 2016

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, , ,
1Life Sciences Solutions,Thermo Fisher Scientific

Capítulos

  • 00:05Título
  • 00:47Induced Pluripotent Stem Cell (iPSC) Generation (d6)
  • 02:14Fibroblast Reprogramming Live Cell Imaging (d19 – 21)
  • 04:16Flow Cytometric Reprogramming Kinetics Measurement
  • 06:35Results: Representative Reprogramming Progression Monitoring
  • 08:10Conclusion

Summary

Tadução automática

Tadução automática

在本研究中提出的协议描述用于通过使用流式细胞仪分析阳性和阴性的多能干细胞标志物的动力学测定的重编程级数的实时监测的方法。该协议还包括的iPSC产生过程中的形态和标志物或报告表达的基于成像的评估。

Tags

Keywords: Somatic Cell ReprogrammingInduced Pluripotent Stem CellsIPSCReprogramming KineticsReprogramming EfficiencyCell Surface MarkersReal-time MonitoringFibroblastsTransductionFeeder-independentPhase ContrastFluorescent ImagingAntibody Labeling

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