Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Culture of Macrophage Colony-stimulating Factor…
JoVE Journal
Immunology and Infection

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Immunology and Infection
Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages
 

Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages

Article DOI: 10.3791/54244-v • 06:46 min • June 30th, 2016
June 30th, 2016 •
1, 1
1Experimental Atherosclerosis Section, National Heart, Lung, and Blood Institute, National Institutes of Health

Capítulos

Resumo
Automatic Translation

A protocol is presented for cell culture of macrophage colony-stimulating factor (M-CSF) differentiated human monocyte-derived macrophages. The protocol utilizes cryopreservation of monocytes coupled with their bulk differentiation into macrophages. Then harvested macrophages can then be seeded into culture wells at required cell densities for carrying out experiments.

Tags

Macrophage Colony-stimulating Factor Monocyte-derived Macrophages MCSF-induced Differentiation Human Macrophage Biology Mononuclear Cells Leukapheresis Counterflow Centrifugal Elutriation Hemocytometer Polypropylene Tube FBS Dimethyl Sulfoxide Cryofile Cell Freezing Container Liquid Nitrogen Cyrofile Tank
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter