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Combining Intravital Fluorescent Microscopy (IVFM) with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Combining Intravital Fluorescent Microscopy (IVFM) with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches

Combining Intravital Fluorescent Microscopy (IVFM) with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches

DOI:
10.3791/54253-v

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11:06 min

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March 21, 2017

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, , ,
1Wells Center for Pediatric Research, Department of Pediatrics,Indiana University School of Medicine, 2Indiana Center for Biological Microscopy, Department of Medicine,Indiana University School of Medicine, 3Department of Pediatrics,Indiana University School of Medicine

Capítulos

  • 00:05Título
  • 01:26Donor Lysozyme Promoter (Lys)-EGFP Mouse Bone Marrow Cell Preparation for Bone Marrow Cell Transplantation (BMT)
  • 03:16Lys-GFP BMT and Intravital Imaging Preparation
  • 05:23In Vivo High Resolution Mouse Calvarium Imaging
  • 07:15Quantitative Analysis
  • 08:14Results: Representative Kinetics and Localization of Myeloid Cell Engraftment Following BMT
  • 10:10Conclusion

Summary

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Intravital fluorescence microscopy (IVFM) of the calvarium is applied in combination with genetic animal models to study the homing and engraftment of hematopoietic cells into bone marrow (BM) niches.

Tags

Intravital Fluorescent MicroscopyIVFMHematopoietic Cell EngraftmentBone Marrow NichesHematopoietic Cell RegenerationCell-to-cell InteractionsMyeloproliferative DiseaseNotch SignalingEGFP MouseBone Marrow TransplantCell IsolationCell SuspensionTail Vein Injection

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