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间隙连接通讯：天然产物的生物标志物的功能，以评估毒物和毒素的不利影响，以及健康的益处
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Biologia
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JoVE Journal Biologia
Gap Junctional Intercellular Communication: A Functional Biomarker to Assess Adverse Effects of Toxicants and Toxins, and Health Benefits of Natural Products
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间隙连接通讯：天然产物的生物标志物的功能，以评估毒物和毒素的不利影响，以及健康的益处

DOI:
10.3791/54281-v

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05:27 min

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December 25, 2016

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, ,
1Department of Pediatrics & Human Development, Institute for Integrative Toxicology,Michigan State University, 2RECETOX — Research Centre for Toxic Compounds in the Environment, Faculty of Science,Masaryk University

Capítulos

  • 00:05Título
  • 00:50SL-DT Bioassay
  • 02:49Loading the Dye
  • 04:17Results: Visualization and Analysis of Toxicant-induced GJIC Inhibition
  • 04:59Conclusion

Summary

Tadução automática

Tadução automática

本协议描述，通过间隙连接通道测量细胞间通讯手术刀装载荧光染料转移技术。间隙连接通讯是一个主要的细胞过程，其中组织稳态维持这种细胞信号中断了不良的健康影响。

Tags

Keywords: Gap Junctional Intercellular CommunicationToxicologyToxicantsToxinsNatural ProductsIn VitroPharmacologyBiomarkerWB-F344 Rat Liver Epithelial CellsDose-responseTime-responsePhenanthreneAcetonitrileCell CultureCell ConfluenceCell Treatment

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