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ガラスの表面機能を介して、標的細胞の単離方法

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A Method of Targeted Cell Isolation via Glass Surface Functionalization

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ガラスの表面機能を介して、標的細胞の単離方法

DOI:

10.3791/54315-v

•

10:40 min

•

September 20, 2016

•

Ali Ansari, Reema Patel, Kinsey Schultheis, Vesna Naumovski, P. I. Imoukhuede

¹Department of Bioengineering,University of Illinois at Urbana-Champaign, ²Department of Liberal Arts & Sciences,University of Illinois at Urbana-Champaign, ³Department of Biomedical Engineering,Illinois Institute of Technology

Capítulos

00:05Título
01:03APTES and DSB Functionalization
03:58Streptavidin Functionalization
04:58Cell Capture and Release
07:36Results: Functionalized Cell Capture of MCF7GFP and RAW Macrophage Cells
09:18Conclusion

Summary

Tadução automática

This protocol describes customizable surface functionalization of the desthiobiotin, streptavidin, and APTES system in order to isolate specific cell types of interest. In addition, this manuscript covers the applications, optimization, and verification of this process.

Tags

Cell Isolation Glass Surface Functionalization Tumor Angiogenesis Cell Biomarkers Drug Resistance Reversible Cell Capture APTES Desthiobiotin EDC MES Buffer Cell Purification Personalized Treatment Cell Type Specific Antibody Oxygen Plasma Silane Oven Cell Release Mechanism

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