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A Method of Targeted Cell Isolation via Glass Surface Functionalization
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Cancer Research
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JoVE Journal Cancer Research
A Method of Targeted Cell Isolation via Glass Surface Functionalization

A Method of Targeted Cell Isolation via Glass Surface Functionalization

DOI:
10.3791/54315-v

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10:40 min

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September 20, 2016

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, , , ,
1Department of Bioengineering,University of Illinois at Urbana-Champaign, 2Department of Liberal Arts & Sciences,University of Illinois at Urbana-Champaign, 3Department of Biomedical Engineering,Illinois Institute of Technology

Chapters

  • 00:05Title
  • 01:03APTES and DSB Functionalization
  • 03:58Streptavidin Functionalization
  • 04:58Cell Capture and Release
  • 07:36Results: Functionalized Cell Capture of MCF7GFP and RAW Macrophage Cells
  • 09:18Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes customizable surface functionalization of the desthiobiotin, streptavidin, and APTES system in order to isolate specific cell types of interest. In addition, this manuscript covers the applications, optimization, and verification of this process.

Tags

Keywords: Cell IsolationGlass Surface FunctionalizationTumor AngiogenesisCell BiomarkersDrug ResistanceReversible Cell CaptureAPTESDesthiobiotinEDCMES BufferCell PurificationPersonalized TreatmentCell Type SpecificAntibodyOxygen PlasmaSilaneOvenCell Release Mechanism

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