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Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering
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JoVE Journal Bioengenharia
Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

DOI:
10.3791/54327-v

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10:27 min

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July 10, 2016

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, , , ,
1Lee Kong Chian School of Medicine,Nanyang Technological University, 2School of Chemical and Biomedical Engineering,Nanyang Technological University, 3NTU-Northwestern Institute of Nanomedicine,Nanyang Technological University

Capítulos

  • 00:05Título
  • 01:16Nanoparticle Labeling of Mesenchymal Stem Cells and Monocytes
  • 03:29Microfluidic Device Fabrication
  • 06:17Operating the Microfluidic Device
  • 09:04Results: Dean Flow Fractionation of Nano-particle Labeled Monocytes (THP-1)
  • 09:59Conclusion

Summary

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Tadução automática

This protocol describes the use of an inertial microfluidics-based buffer exchange strategy to purify micro/nanoparticle engineered cells with efficient depletion of unbound particles.

Tags

Keywords: MicrofluidicBuffer ExchangeNanoparticleCell EngineeringInertial FocusingSpiral MicrochannelMesenchymal Stem CellsTHP-1 CellsSilica NanoparticlesPLGA Calcium NanoparticlesPoly-l-lysineCell LabelingCell PurificationHigh ThroughputRegenerative Medicine

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