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微流控缓冲液更换为无干扰微/纳米颗粒细胞工程

JoVE Journal
Bioengenharia

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JoVE Journal Bioengenharia

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

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微流控缓冲液更换为无干扰微/纳米颗粒细胞工程

DOI:

10.3791/54327-v

•

10:27 min

•

July 10, 2016

•

Hui Min Tay, David C. Yeo, Christian Wiraja, Chenjie Xu³, Han Wei Hou

¹Lee Kong Chian School of Medicine,Nanyang Technological University, ²School of Chemical and Biomedical Engineering,Nanyang Technological University, ³NTU-Northwestern Institute of Nanomedicine,Nanyang Technological University

Capítulos

00:05Título
01:16Nanoparticle Labeling of Mesenchymal Stem Cells and Monocytes
03:29Microfluidic Device Fabrication
06:17Operating the Microfluidic Device
09:04Results: Dean Flow Fractionation of Nano-particle Labeled Monocytes (THP-1)
09:59Conclusion

Summary

Tadução automática

这个协议描述了使用惯性基于微流体缓冲交换策略的纯化微/纳米工程细胞与未结合的颗粒的有效消耗。

Tags

Keywords: Microfluidic Buffer Exchange Nanoparticle Cell Engineering Inertial Focusing Spiral Microchannel Mesenchymal Stem Cells THP-1 Cells Silica Nanoparticles PLGA Calcium Nanoparticles Poly-l-lysine Cell Labeling Cell Purification High Throughput Regenerative Medicine

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