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Imunologia e Infecção

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制备及胸腺器官切片文化中的应用

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Preparation and Applications of Organotypic Thymic Slice Cultures

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制备及胸腺器官切片文化中的应用

DOI:

10.3791/54355-v

•

10:10 min

•

August 06, 2016

•

Aditi Sood*^1,2, Mengqi Dong*^1,2, Heather J. Melichar³

¹Centre de Recherche,Hôpital Maisonneuve-Rosemont, ²Department of Microbiology, Infectiology and Immunology,Université de Montréal, ³Department of Medicine,Université de Montréal

Capítulos

00:05Título
00:45Harvest of Mouse Thymus
01:52Agarose Embedding and Vibratome Sectioning of Thymic Lobes
05:30Preparation of Thymocytes for Overlaying
06:59Overlaying Thymocytes onto the Thymic Slices
07:48Results: Positive and Negative Selection of Thymocytes
09:29Conclusion

Summary

Tadução automática

我们描述的是，在用流式细胞仪组合，可以被用来建模显影T细胞的阳性和阴性选择胸腺切片的制备方法。胸腺片也可以适用于胸腺细胞迁移，本地化的原位分析，并通过免疫荧光和双光子显微镜信令。

Tags

Keywords: Organotypic Thymic Slice Cultures T Cell Development Positive And Negative Selection Thymic Cortex And Medulla Thymocyte Subsets Thymic Microenvironment Mouse Thymus Dissection Agarose Embedding RPMI-1640 Media Cell Culture Inserts

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