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In Vivo Study of Human Endothelial-Pericyte Interaction Using the Matrix Gel Plug Assay in Mouse
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Biologia
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JoVE Journal Biologia
In Vivo Study of Human Endothelial-Pericyte Interaction Using the Matrix Gel Plug Assay in Mouse

In Vivo Study of Human Endothelial-Pericyte Interaction Using the Matrix Gel Plug Assay in Mouse

DOI:
10.3791/54617-v

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08:16 min

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December 19, 2016

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, , ,
1Division of Pulmonary and Critical Care Medicine, School of Medicine,Stanford University, 2Stanford Cardiovascular Institute, School of Medicine,Stanford University, 3VA Palo Alto Health Care System, Department of Cardiothoracic Surgery, School of Medicine,Stanford University

Capítulos

  • 00:05Título
  • 00:56Cell and Matrix Gel Preparation
  • 03:24Matrix Gel Injection
  • 05:05Plug Isolation: 14 Days Post-injection
  • 06:16Result: H&E and Immunofluorescent Staining
  • 07:00Conclusion

Summary

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We present a protocol to study human endothelial-pericyte interactions in mouse using a variation of the matrix gel plug angiogenesis assay.

Tags

In VivoEndothelial-pericyte InteractionMatrix Gel Plug AssayMouseAngiogenesisBlood Vessel FormationSignaling PathwaysVascular DisordersEndothelial CellsPericytesCell CultureMatrigelBeta-FGFTrypsinizationCell CountingCell Injection

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