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JoVE Journal Biochemistry
Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase
 

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase

Article DOI: 10.3791/54652-v • 11:01 min • November 23rd, 2016
November 23rd, 2016 •
1, 1, 1, 1, 1, 1, 1, 1,2
1Drug Discovery and Development, Istituto Italiano di Tecnologia, 2Departments of Anatomy and Neurobiology, Pharmacology, and Biological Chemistry, University of California, Irvine School of Medicine

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Here, we describe the preparation and use of an activity-based probe (ARN14686, undec-10-ynyl-N-[(3S)-2-oxoazetidin-3-yl]carbamate) that allows for the detection and quantification of the active form of the proinflammatory enzyme N-acylethanolamine acid amidase (NAAA), both in vitro and ex vivo.

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Keywords: Activity-based Probe N-acylethanolamine Acid Amidase (NAAA) Synthesis In Vivo Use Fluorescence Microscopy Inflammatory Diseases Neurodegenerative Diseases Undecynenol DMAP DPC Dichloromethane Sodium Bicarbonate Sodium Sulfate Oxo Asetydenol Ammonium Asetate Diazapropalethylamine
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