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Immunology and Infection

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JoVE Journal Immunology and Infection
Assays for Studying the Role of Vitronectin in Bacterial Adhesion and Serum Resistance
 

Assays for Studying the Role of Vitronectin in Bacterial Adhesion and Serum Resistance

Article DOI: 10.3791/54653-v • 10:55 min • October 16th, 2018
October 16th, 2018 •
1,2, 1, 1, 1, 1
1Clinical Microbiology, Department of Translational Medicine, Lund University, 2Department of Molecular Biology, Umea University

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Resumo
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This report describes protocols for characterizing interactions between bacterial outer membrane proteins and the human complement regulator vitronectin. The protocols can be used to study the binding reactions and biological function of vitronectin in any bacterial species.

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Keywords: Vitronectin Bacterial Adhesion Serum Resistance Host-pathogen Interaction Complement Inhibitor Flow Cytometry ELISA Antihuman Vitronectin Antibody FITC-conjugated Antibody Recombinant PH Protein
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