Journal
/
Biologia
/
Visualizing Stromule Frequency with Fluorescence Microscopy
JoVE Journal
Biologia
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia
Visualizing Stromule Frequency with Fluorescence Microscopy

Visualizing Stromule Frequency with Fluorescence Microscopy

DOI:
10.3791/54692-v

•

08:27 min

•

November 23, 2016

•

, ,
1Plant Gene Expression Center, Agricultural Research Service,USDA, 2Department of Plant and Microbial Biology,University of California, Berkeley

Capítulos

  • 00:05Título
  • 00:53Preparing Leaf Samples for Visualization
  • 01:53Visualizing Stromules with Confocal Fluorescence Microscopy and Image Processing
  • 03:28Extracting Intact Chloroplasts to Visualize Stromule Dynamics
  • 05:58Results: Quantifying Stromule Frequency in N. benthamiana Leaves
  • 07:22Conclusion

Summary

Tadução automática

Tadução automática

Protocols to investigate the dynamics of chloroplast stromules, the stroma-filled tubules that extend from the surface of chloroplasts, are described.

Tags

ChloroplastStromuleFluorescence MicroscopyLive Cell ImagingNicotiana BenthamianaLeaf Sample PreparationChloroplast IsolationCytoskeletonZ-stackGFPConfocal Microscopy

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/54692?article_type=v

Visualizing the Live Drosophila Glial-neuromuscular Junction with Fluorescent Dyes
check_url/pt/54692?article_type=v

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy
check_url/pt/54692?article_type=v

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
check_url/pt/54692?article_type=v

Time-lapse Imaging of Mitosis After siRNA Transfection
check_url/pt/54692?article_type=v

Cell Electrofusion Visualized with Fluorescence Microscopy
check_url/pt/54692?article_type=v

Visualizing Bacteria in Nematodes using Fluorescent Microscopy
check_url/pt/54692?article_type=v

Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy
check_url/pt/54692?article_type=v

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
check_url/pt/54692?article_type=v

Rapid Analysis and Exploration of Fluorescence Microscopy Images
check_url/pt/54692?article_type=v

Analysis of Autophagy in Penicillium chrysogenum by Using Starvation Pads in Combination With Fluorescence Microscopy

Read Article