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间接神经元的星形胶质细胞共培养试验：一个<em>体外</em>设置 - 为神经元的神经胶质细胞相互作用的详细调查

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Neurociência

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JoVE Journal Neurociência

The Indirect Neuron-astrocyte Coculture Assay: An In Vitro Set-up for the Detailed Investigation of Neuron-glia Interactions

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间接神经元的星形胶质细胞共培养试验：一个<em>体外</em>设置 - 为神经元的神经胶质细胞相互作用的详细调查

DOI:

10.3791/54757-v

•

11:08 min

•

November 14, 2016

•

Christine Gottschling, Egor Dzyubenko, Maren Geissler, Andreas Faissner

¹Department of Cell Morphology and Molecular Neurobiology, Faculty of Biology and Biotechnology,Ruhr-University

Capítulos

00:05Título
02:01Preparation and Cultivation of Primary Cortical Astrocytes
05:19Preparing Astrocytes for the Indirect Coculture
06:48Preparation of Primary Hippocampal Neurons
09:03Results: The Indirect, Astrocyte-neuron Coculture System Allows for Separate Analysis of Astrocytes, Neurons and Secreted Molecular Mediators
10:01Conclusion

Summary

Tadução automática

该协议描述了神经元的神经胶质细胞相互作用的条块分析间接神经元的星形胶质细胞共培养。

Tags

Keywords: Indirect Neuron-astrocyte Coculture Assay Neuron-glia Interactions Synapse Formation Astrocyte Secretome Primary Astrocytes Primary Embryonic Neurons Permeable Membrane

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