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第一次混合胶质细胞培养的成年小鼠脊髓组织准备
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Neurociência
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JoVE Journal Neurociência
Preparation of Primary Mixed Glial Cultures from Adult Mouse Spinal Cord Tissue
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第一次混合胶质细胞培养的成年小鼠脊髓组织准备

DOI:
10.3791/54801-v

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07:13 min

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November 19, 2016

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,
1Department of Biomedical Sciences, College of Osteopathic Medicine,University of New England

Capítulos

  • 00:05Título
  • 00:55Preparation of the Single Cell Suspension
  • 03:12Removal of Myelin from the Single Cell Suspension
  • 05:03Results: Mixed Glial Cultures and Response to Stimuli
  • 06:11Conclusion

Summary

Tadução automática

Tadução automática

神经性疼痛的发展涉及脊髓神经胶质细胞的病理变化。从成年脊髓组织的，设计用于在体外研究这些细胞中的胶质可靠的培养体系缺乏。因此，我们在这里展示了如何建立从成年小鼠的脊髓组织的主要胶质细胞混合培养。

Tags

Keywords: Primary Mixed Glial CulturesAdult Mouse Spinal CordIn Vitro StudiesNeuropathic PainMultiple SclerosisHBSSPapain DNAseEnzyme DigestionSingle Cell SuspensionAlbumin Ovomucoid InhibitorDNAseCentrifugationMyelin Removal

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