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JoVE Journal Biochemistry
Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
 

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Article DOI: 10.3791/55070-v • 08:09 min • January 7th, 2017
January 7th, 2017 •
1, 1
12bind GmbH

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MicroScale Thermophoresis (MST) is a sensitive technology to characterize aptamer-target interactions. This manuscript describes an MST protocol to characterize aptamer-small molecule interactions.

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Binding Site Aptamer ATP Microscale Thermophoresis DNA Aptamer Small Molecule Interactions Binding Parameters Technology Interaction Partner Size Quality Control Data Challenging Interactions Drug-target Interactions Antigen/antibody Interactions Cy5 Labeled DNA DH25.42 Aptamer Distilled Water Binding Buffer Incubate Serial Dilution Ligand Stock Solution
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