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Targeted Plasma Membrane Delivery of a Hydrophobic Cargo Encapsulated in a Liquid Crystal Nanoparticle Carrier
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JoVE Journal Bioengenharia
Targeted Plasma Membrane Delivery of a Hydrophobic Cargo Encapsulated in a Liquid Crystal Nanoparticle Carrier

Targeted Plasma Membrane Delivery of a Hydrophobic Cargo Encapsulated in a Liquid Crystal Nanoparticle Carrier

DOI:
10.3791/55181-v

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10:16 min

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February 08, 2017

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, , , ,
1Center for Biomolecular Science and Engineering,Naval Research Laboratory, 2Optical Sciences Division,Naval Research Laboratory, 3Sotera Defense Solutions

Capítulos

  • 00:05Título
  • 00:51Conjugation of PEG-Chol to DiO-LCNP via EDC Coupling and Characterization of the Product
  • 02:33Preparation of Cell Culture Dishes for Delivery Experiments and Imaging
  • 04:07Cellular Delivery of DiO and DiO-LCNPs and Imaging of Fixed Cells
  • 06:11Cellular Delivery DiO-LCNPs and DiI FRET Imaging in Live Cells
  • 07:37Results: Analysis of DiO-LCNP-PEG-Chol Cellular Uptake to the Plasma Membrane and Quantification of Cytotoxicity
  • 08:57Conclusion

Summary

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A liquid crystal nanoparticle (LCNP) nanocarrier is exploited as a vehicle for the controlled delivery of a hydrophobic cargo to the plasma membrane of living cells.

Tags

Liquid Crystal NanoparticleHydrophobic CargoPlasma Membrane DeliveryPEG Cholesterol ConjugationGel ElectrophoresisHEK 293 T17 Cell Culture

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