Journal
/
Imunologia e Infecção
/
系统抑制剂靶向细胞内的疗效和毒性筛选<em>结核分枝杆菌</em
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis
Please note that all translations are automatically generated. Click here for the English version.

系统抑制剂靶向细胞内的疗效和毒性筛选<em>结核分枝杆菌</em

DOI:
10.3791/55273-v

•

09:57 min

•

April 05, 2017

•

,
1Department of Medicine,University of British Columbia

Capítulos

  • 00:05Título
  • 00:58THP-1 Medium and Maintenance and High-throughput Intracellular Screening Using Luciferase-expressing M. tuberculosis H37Rv
  • 03:54Cytotoxicity Analysis Using an MTT Assay
  • 06:06In-broth Activity Analysis Using a Resazurin Assay
  • 07:16Results: Efficacy Screening of Inhibitors of M. tuberculosis
  • 08:53Conclusion

Summary

Tadução automática

Tadução automática

我们已经开发了模块化高通量筛选系统，用于发现新化合物对结核分枝杆菌 ，靶向细胞内和细胞在肉汤中生长的细菌以及细胞毒性对哺乳动物宿主细胞。

Tags

Mycobacterium TuberculosisIntracellular InfectionMacrophagesLuciferase AssayCytotoxicityMICDrug DevelopmentTHP1 CellsOpsonizationMOIDifferentiation96-well PlateCompound Screening

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/55273?article_type=v

样品制备<em>结核分枝杆菌</em提取物对核磁共振的代谢组学研究
check_url/pt/55273?article_type=v

增长结核分枝杆菌生物膜
check_url/pt/55273?article_type=v

实验模型后的自然传播到研究结核病，疟疾混合感染结核分枝杆菌和伯氏疟原虫</i
check_url/pt/55273?article_type=v

高通量筛选RNA病毒的广谱化学抑制剂
check_url/pt/55273?article_type=v

展现着多药耐药<em>结核分枝杆菌</em>扩增基因芯片
check_url/pt/55273?article_type=v

HIV逆转录酶和整合酶抑制剂的快速筛选
check_url/pt/55273?article_type=v

关于功能研究的三维人体肺组织模型<em>结核分枝杆菌</em>感染
check_url/pt/55273?article_type=v

高通量兼容检测，以评估对巨噬细胞传代药物疗效<em>结核分枝杆菌</em
check_url/pt/55273?article_type=v

分析18葡萄糖 PET/CT 成像作为研究结核分枝杆菌感染和治疗非人类灵长类动物的工具
check_url/pt/55273?article_type=v

报告酶荧光法检测小鼠结核分枝杆菌的影像学

Read Article