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Mammalian Cell Encapsulation in Alginate Beads Using a Simple Stirred Vessel
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Capítulos
- 00:00Título
- 00:10Introduction
- 02:49Preparation of Alginate Solution, CaCO3 Suspension and Acidified Oil
- 04:56Alginate Bead Generation by Emulsion and Internal Gelation
- 06:45Bead Recovery
- 08:19Representative Cell Immobilization Results
- 09:11Conclusion
This video and manuscript describe an emulsion-based method to encapsulate mammalian cells in 0.5% to 10% alginate beads which can be produced in large batches using a simple stirred vessel. The encapsulated cells can be cultured in vitro or transplanted for cellular therapy applications.
