/

/

使用简单搅拌容器在藻酸盐珠中的哺乳动物细胞包封

JoVE Journal
Bioengenharia
Author Produced

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Bioengenharia

Mammalian Cell Encapsulation in Alginate Beads Using a Simple Stirred Vessel

Please note that all translations are automatically generated. Click here for the English version.

使用简单搅拌容器在藻酸盐珠中的哺乳动物细胞包封

DOI:

10.3791/55280-v

•

10:20 min

•

June 29, 2017

•

Corinne A. Hoesli, Roger L. J. Kiang, Kamini Raghuram, René G. Pedroza, Karen E. Markwick, Antonio M. R. Colantuoni, James M. Piret

¹Department of Chemical Engineering,McGill University, ²Michael Smith Laboratories & Department of Chemical and Biological Engineering,University of British Columbia, ³Michael Smith Laboratories & Department of Pharmaceutical Sciences,University of British Columbia

Capítulos

00:00Título
00:10Introduction
02:49Preparation of Alginate Solution, CaCO₃ Suspension and Acidified Oil
04:56Alginate Bead Generation by Emulsion and Internal Gelation
06:45Bead Recovery
08:19Representative Cell Immobilization Results
09:11Conclusion

Summary

Tadução automática

该视频和手稿描述了一种基于乳液的方法，以将哺乳动物细胞包封在0.5％至10％的藻酸盐珠中，其可以使用简单的搅拌容器以大批量生产。包封的细胞可以在体外培养或移植用于细胞治疗应用。

Tags

Keywords: Cell Encapsulation Alginate Beads Stirred Vessel Emulsion Internal Gelation Calcium Carbonate Acetic Acid Immunoisolation Transplantation Scalability Robustness Droplet Size Mechanical Properties Cell Survival

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

使用介电泳分离珠和多通道微流控芯片细胞和层流

卵泡在蛋白质降解的3维系统的封装和文化的一种方法

蛋白质和细胞坐月子创建二维图案衬底

心肌在心脏组织工程中的纤维蛋白凝胶的封装

海藻酸钠微胶囊作为一个人类胚胎干细胞（胚胎干细胞）的繁殖和分化的3D平台，以不同的谱系

在一个容器的支架部署在监测的墙力学

高吞吐量的单细胞和多细胞微胶囊

新方法本地化记者荧光珠近细胞培养表面的牵引力显微镜

培养哺乳动物细胞使用一次性使用气动生物反应器系统

分层海藻酸钠构造：一个异质细胞群的共培养平台

Read Article