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方法来研究上皮转运蛋白功能和表达在纯肠和Caco-2细胞生长在3D
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Biologia
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JoVE Journal Biologia
Methods to Study Epithelial Transport Protein Function and Expression in Native Intestine and Caco-2 Cells Grown in 3D
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方法来研究上皮转运蛋白功能和表达在纯肠和Caco-2细胞生长在3D

DOI:
10.3791/55304-v

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11:27 min

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March 16, 2017

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, , , , , , , ,
1Department of Medicine,University of Illinois at Chicago, 2Department of Research,Jesse Brown VA Medical Center

Capítulos

  • 00:05Título
  • 01:533D Caco-2 Cell Culture on a Gelatinous Protein Solution
  • 03:23Immunofluorescence Staining of Epithelial Transporters in 3D Caco-2 Cells
  • 05:12Measurement of 5-HT Uptake in Mouse Ileum
  • 06:29Equilibration and Tissue Treatment
  • 07:41Quantification of the Mucosal to serosal Flux (Jm-s) and 3[H]-5-HT Accumulation
  • 08:42Results: The Expression and Function of SERT Protein Measured in Caco-2 3D Cell Cultures and Ileal Mucosa Ex Vivo
  • 09:57Conclusion

Summary

Tadução automática

Tadução automática

我们描述了简单的方法来研究肠血清素转运蛋白（SERT）的功能和表达的使用体外细胞培养的Caco-2细胞在三维生长模型和小鼠肠的离体模型的调节。这些方法适用于其他上皮转运的研究。

Tags

Epithelial TransportSerotonin TransporterSERTCaco-2 Cells3D Cell CultureUssing ChamberIntestinal EpitheliumElectrolyte TransportersCell-cell InteractionsCell-extracellular Matrix InteractionsTransport FunctionTherapeutic Screening

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